생물리화학 실험실

1) Assembly of the NPC and the Mediator Complex from their ~30 distinct subunits. 2) Interactions of the NPC with nucleocytoplasmic transport receptors and of the Mediator complex with transcription factors. In parallel, we aim to devise cellular systems to probe the effects of such conformational changes on nucleocytoplasmic transport and transcription in vivo. Given the abundance of IDRs in the eukaryotic proteome, the molecular principles and quantitative methods established in the proposed research will be applicable to other complex biological systems. These basic studies will also provide invaluable information for future medical investigations, culminating in innovative therapeutic approaches targeting IDRs in human diseases.