3D 바이오가공 연구실

Adipose tissue is one of the most widely implanted tissue for therapeutics and reconstruction. The tissue contains complex extracellular matrix (ECM), adipocyte, stromal vascular fraction (SVF) and etc. However, clinical outcomes of implantation demonstrate prognosis that caused sclerosis and immune-related disease. Our research aims to develop adipose tissue with heterogeneous tissue composition and enhanced survival rate for successful implantation. Vasculature is an essential element in artificial tissues and disease model designs. To fabricate desired microvasculature, we designed the multi-cellular construct with 3D bioprinting technology. Two vascular channels were formed with directly printing an endothelial cells-laden bioink. And the capillary network was induced by gradient of angiogenic factors(ex. VEGF) by co-printing the supporting cells. To control the capillary network pattern, different concentrations of hydrogel were patterned to differentiate the diffusion rate of angiogenic factors and matrix stiffness within the structure. Using our strategy, micro-vascular network can be induced in desired pattern modulating the construct design. We demonstrated the feasibility of our vascular patterning technique by engineering various patterns of micro-vascular network. Also, the functionality of induced capillary network was confirmed with dextran infiltration test and observation of the lumen structure. Our bioprinting technique has a great potential to construct bio-mimetic vascular networks within 3D artificial tissues. According to the needs of precision medicine, the development of a patient-specific drug delivery system (DDS) is becoming essential. In the pharmaceutical industry, 3D printing is a useful manufacturing technique to fabricate tailored controll